The use of B-ultrasound guided collection of bovine oocytes and the application of bovine embryo transfer technology have greatly improved the reproductive efficiency of cows. However, due to the instability of cows' response to exogenous hormone superovulation, especially the adverse effects of continuous superovulation treatment on donor cows, obtaining multiple embryos from high-quality cows is limited.

However, with the maturation of bovine in vitro fertilization, embryos can be produced in vitro, but the required oocytes are mainly obtained from slaughtered bovine ovaries. In China, ovaries obtained from slaughterhouses have no breeding value. Although the recovery rate of oocytes using endoscopic egg retrieval method is high, it can easily cause damage to the vagina and ovaries, and the operation of endoscopy is somewhat difficult. ultrasonic egg retrieval method is simple, reliable, and minimally damaging to the vagina, and can be used for egg retrieval on any day of the cow's sexual cycle.

The current live egg retrieval technology mainly relies on animal B-ultrasound guidance. Since the successful use of animal B-ultrasound scanning devices to explore the collection of live bovine oocytes through the bovine vagina in 1988, this technology has given more practical significance to related biotechnologies such as bovine in vitro fertilization. Applying this technology to obtain oocytes for bovine in vitro fertilization to produce embryos can greatly promote the application of bovine in vitro fertilization technology in animal husbandry production and achieve industrialization.

The application of live egg harvesting technology can continuously supply eggs to high-yielding cows and high-quality beef cattle that are under reproductive age, have poor superovulation effects, or are elderly. Combined with relevant biotechnology, it can shorten the breeding time, fully tap the reproductive potential of high-quality cows, and mass produce high-quality embryos, And it can further provide necessary materials for egg nuclear transfer, gene transfer, etc.