Contrast agents and various ultrasound scanning modes can display dynamic echo enhancement changes of different lesions during intermittent or continuous acoustic scanning. Most ultrasound contrast agents alter the basic interaction between sound waves and tissues, namely absorption, reflection, and refraction, by changing sound attenuation, sound velocity, and enhanced backscatter, thereby enhancing the echo signal in the affected area. Hydrogen peroxide, as a contrast agent, has been applied in clinical practice. The B-ultrasound imaging effect of hydrogen peroxide on bovine liver was observed in cattle, and the action time and effect were observed under different concentrations of hydrogen peroxide.

B-ultrasound images of the liver of three experimental cows under natural conditions; Real time recording of liver ultrasound images of three experimental cows after intravenous infusion of 0.5 concentration hydrogen peroxide (100mL) immediately prepared via the jugular vein for 3-50min; 48 hours after the end of the second experiment, real-time recording of changes in liver ultrasound images of three experimental cows after intravenous infusion of 1.0 concentration hydrogen peroxide (100 mL) immediately prepared into the jugular vein was performed using animal ultrasound for 3-50 minutes; 48 hours after the completion of the third experiment, real-time recording of changes in liver ultrasound images of three experimental cows after intravenous infusion of 1.5% concentration hydrogen peroxide (100mL) immediately prepared via jugular vein was performed for 3-50 minutes.

The ultrasound images of bovine liver under natural conditions and after injection of different concentrations of hydrogen peroxide are shown in Figures 1-8. From the picture, it can be seen that the ultrasound images of liver parenchyma, hepatic vein wall, and diaphragm with injected hydrogen peroxide show enhanced echoes, which are more pronounced than normal. Hydrogen peroxide with contrast enhancement rates of 100mL, 0.5%, 1.0%, and 1.5% can significantly enhance liver echoes, with a contrast enhancement rate of 100%. The time difference between the appearance of 100mL 0.5, 1.0%, and 1.5 hydrogen peroxide after intravenous infusion is not significant. Hydrogen peroxide can be observed in the liver within 2-3 minutes, but the duration increases with increasing concentration.

Three concentrations of hydrogen peroxide contrast showed small, dense, and bright light spots rapidly moving towards the distal end of the hepatic vein lumen, quickly distributed throughout the liver parenchyma as veins. Among them, when 1.0% hydrogen peroxide was used for contrast imaging, the liver showed a dendritic network like echo, and the entire liver quickly presented a uniform strong echo. The boundary between liver structure and surrounding tissues was the most obvious.