Veterinary Ultrasound diagnosis is of great significance to improving the management level of animal husbandry and promoting the economic development of animal husbandry. Using veterinary ultrasound diagnosis technology to evaluate the reproductive capacity of buffaloes can help us understand and recognize the status of buffalo ovaries and uterus during estrus cycle and pregnancy, and better improve reproductive performance and promote animal genetic process.

Under the fluorescent screen display, use the probe of veterinary ultrasound to determine the follicle. Push the puncture needle from the puncture stand along the direction of the puncture line and pierce the follicle. Pedal the low-pressure suction device to suck the oocyte into the oviduct. The oocyte can be fertilized after in vitro maturation and can also be used for embryo transplantation. In 1993, Boni et al. in Italy first reported the study of live egg collection of buffaloes. In 1998, Galli et al. used 2 dry-milk female buffaloes to collect live oocytes and combined with in vitro fertilization technology to produce in vitro embryos. Then 9 frozen embryos were thawed and transplanted to 9 recipient cows in estrus at the same time. Finally, 3 cows were confirmed to be pregnant and all developed to the full term of pregnancy. The first successful birth of three surviving buffalo calves. The research on the live egg collection technology of buffalo using B-ultrasound instrument in my country started late. Huang Youjun et al. (2004) reported that 42 river-type buffaloes were live egg-collected using B-ultrasound egg-collector. Eggs were collected once every 3 days. Eggs were collected 2-3 times in succession. A total of 96 buffaloes were collected. 762 follicles were punctured and 513 oocytes were recovered. The recovery rate was 67.3%. Among them, 305 oocytes were usable. The usable rate was 59.5%: an average of 5.34-4.25 oocytes were obtained per head. Among them, 3.18-2.89 oocytes were usable. The usable oocytes were cultured in two systems, cell co-culture and synthetic oviduct fluid culture (SOF). The results showed that the fertilization cleavage rate and blastocyst rate were 45.2%, 30.6% and 70.8%, 18.8%, respectively. Eight embryos were transplanted into eight swamp-type recipient cows. Two were confirmed to be pregnant and all developed to full term. The world's first river buffalo bred from a swamp-type buffalo was born.